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The ǧ����Ƶ Zebrafish Facility is located in Rowe 111. The Facility provides zebrafish husbandry, breeding and egg production services. MBL partners with zebrafish life support system vendors who showcase their different system solutions within the Facility. This includes systems from Aquaneering, Inc., Tecniplast and Aquarius Fish Systems. These systems provide our operational capacity for supporting Investigators and courses who utilize zebrafish and provides them with opportunities to evaluate these solutions for their own needs. The systems are kept at a constant temperature of 28°C and water quality is monitored twice each day. All systems are alarmed, and a maintenance person is on-call 24 hrs/day to ensure safe keeping of all fish. The photoperiod for the facility is lights “on” at 9:00 AM and “off” at 11:00 PM.

Wild type fertilized embryos are available year-round. Specific mutant lines are also available at various times of the year. Investigators wishing to obtain embryos are required to have IACUC protocols approved prior to placing their order. Requests for eggs/embryos must be made at least 48 hours prior to the date they will be needed. Specific cell stages are available with coordination with the facility supervisor.

Please contact Zebrafish Facility Supervisor, Troy McInerney (tpmcinerney@mbl.edu) for any zebrafish service needs.