CMF Equipment

The Central Microscopy Facility supply year round and summer investigators as well as courses with instrumentation, some owned by MBL and some on loan, so please read through this guide to familiarize yourself with the Facility. Feel free to contact the Facility personnel for more information. Some of the CMF rooms are on the ǧƵ One Card access system. You will need to get CMF authorization for your card to work on those CMF doors.

Scheduling Time and Trainings:
We use an on-line system by Stratocore for scheduling time and for setting up training sessions on individual microscopy systems. See this page for instructions.

Contact Louis Kerr at Marine Biological Laboratory, Woods Hole, Ma, 02543, Phone:  (508) 289-7273, Email: lkerr@mbl.edu for more information.

Electron Microscopy

TEM JEOL JEM-200CX Microscope
Microscope Name:
TEM JEOL JEM-200CX
General Overview:
80-200KV TEM equipped with:

- Low magnification mode (+/- 60 degrees tilt and 300 degree rotation)
- Standard specimen cartridge and goniometer specimen cartridge
- AMT digital side mount and bottom mount cameras

Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Electron
Detector:
Camera
Location - Building and Room
Lillie 208
Contact person:
Louie Kerr
Hourly Rate for Use:
$35/hr
SEM Zeiss Supra 40VP Microscope
Microscope Name:
SEM Zeiss Supra 40VP
General Overview: The Zeiss Supra 40VP scanning electron microscope provides high resolution surface imaging with three-dimensional appearance. it has a field emission cathode as the electron source and several detectors (SE2, inlens SE, STEM, BSD, variable pressure SE). It is equipped with an EDS (Energy Dispersive Spectrometry) system and an EBSD (Electron Backscatter Diffraction) system.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Electron
Features:
  • 0.1 – 30kV accelerating voltage
  • Oxford Instruments Inca Synergy 350 Energy Dispersive X-ray (EDS) microanalysis system
  • HKL Premium electron backscatter diffraction (EBSD) system
  • Leica cryo preparation and cryo stage components
  • Zeiss Atlas 5 Array Tomography Package
Detector:
Secondary electron (SE2); inlens SE; Scanning Transmission Electron Microscopy (STEM); Backscatter Detector (BSD); variable pressure Mode SE
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
Yes
Location - Building and Room
Lillie 207
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use: $35/hour for standard SEM; Additional $20/hour for cryoSEM and EDS/EBSD or Array tomography

Light Microscopy

lsm710 microscope
Microscope Name:
Zeiss LSM710
General Overview:
The Zeiss LSM 710 NLO is a multi-purpose, visible light confocal laser scanning microscope. It has three independent simultaneous spectral detection channels for reflection and fluorescence and an extra detector for transmitted light. The LSM 710 is configured on an inverted Observer Z1 microscope with a motorized stage along with a variety of objectives and a temperature-controlled stage. The system is capable of confocal imaging using 7 visible lines (405, 458, 488, 514, 561, 594 and 633 nm) as well as two photon excitation using the Coherent Chameleon Vision II laser. The non-descanned system includes a 2 channel GaAsP detector for reflected light and a 2-channel module on the transmitted path.
Commercial or Pre-commercial:
Commercial
Which Facility:
Central Microscopy Facility
Imaging Mode:
Optical
If Optical:
Widefield; Confocal; Multi-photon
Microscope Stand:
Inverted
Features:
Coherent Chameleon laser offers a tuning range from 680nm to 1080nm for 2-photon-imaging
Laser Wavelengths:
405 nm, 458 nm, 488 nm, 561 nm, 594 nm, 640 nm
Excitation/Emission Filters:
GFP (Zeiss 38HE); DsRed (Zeiss 43HE) ; Two-Photon: BS-MP UV/690 ; NDD BS MP-760
Detector:
PMT; NDD (Non-Descanned Detector)
Imaging lenses:
5x ; 10x ; 20x ; 40xOil ; 40xW ; 63xOil (more lenses available) / Optovar: 1x ; 1.25x
Microscope Control software:
ZenBlack 2.3
Temperature control:
Yes
CO2:
Yes
Humidity:
Yes
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room:
Lillie 209
Contact Person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$33/hr (after 6hrs $16.50/hr)
Zeiss LSM 780 microscope
Microscope Name:
Zeiss LSM 780
General Overview:
The Zeiss LSM 780 is a multi-purpose, visible light confocal laser scanning microscope. It is equipped with a 34 channel spectral array with laser lines at 405nm, 458nm, 488nm, 514nm, 561nm, 594nm and 633nm. The LSM 780 is configured on an inverted Observer Z1 microscope with a motorized stage along with a variety of objectives.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield, Confocal
Microscope Stand:
Inverted
Features:
Capability of spectral imaging/linear unmixing
Laser Wavelengths:
405 nm, 458 nm, 488 nm, 561 nm, 594 nm, 640 nm
Excitation/Emission Filters:
DAPI (Zeiss 49) ; GFP (Zeiss 38HE) ; DsRed (Zeiss 43HE)
Detector:
PMT
Imaging lenses:
5x ; 10x ; 20x ; 25xOil ; 100xOil (more lenses available)
Microscope Control software:
ZenBlack2.3
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Additional details:
Location - Building and Room:
Lillie 212
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$33/hr (after 6hrs $16.50/hr)
Sutter MOM 2P System microscope
Microscope Name:
Sutter MOM 2P System
General Overview:
The Movable Objective Microscope (MOM) from Sutter is a two-photon microscope capable of imaging deep within living specimens when combined with an appropriate laser. It provides 3-dimensional objective movement and rotation allowing the specimen to remain horizontal and stationary.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Confocal, Multi-photon
Microscope Stand:
adjustable position (upright or inverted or in-between)
Features:
Objective rotates about optical axis for imaging ; customizable open platform design
Laser Wavelengths:
Coherent Chameleon Ultra2 laser offers a tuning range from 680nm to 1080nm
Detector:
PMT
Imaging lenses:
20xW, 0.8NA ; other lenses available
Microscope Control software:
MScan3.0
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting as well as custom solutions
Location - Building and Room:
Rowe 203
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$33/hr (after 6hrs $16.50/hr)
Zeiss Axio Observer Spinning Disc microscope
Microscope Name:
Zeiss Axio Observer Spinning Disc
General Overview:
The Zeiss Cell Observer Spinning Disk confocal system has a Photometrics Evolve 512×512 camera. It is configured on an inverted Observer Z1 microscope with a motorized stage and a complete incubator chamber system.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield, Confocal
Microscope Stand:
Inverted
Features:
Electrophysiology equipment and FRAP module ; Evolve 512 Delta EMCCD Camera
 
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 635 nm
Excitation/Emission Filters:
DAPI (Zeiss 49) ; GFP (Zeiss 38HE) ; DsRed (Zeiss 43HE) ; BS C/G/DR
Detector:
Camera
Imaging lenses:
10x ; 20x ; 40xOil ; 40xW ; 100xOil ; 150xGly
Microscope Control software:
ZenBlack2
Temperature Control:
Yes
CO2 Control:
Yes
Humidity Control:
Yes
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Additional details:
Location - Building and Room:
Loeb G63
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$28/hr (after 6hrs $14/hr)
Zeiss Lightsheet7 microscope
Microscope Name:
Zeiss Lightsheet7
General Overview:
The Zeiss Lightsheet 7 is a stand-alone SPIM (Selective Plane Illumination Microscope) capable of imaging large, living specimens at much greater speeds than more traditional confocal technology. The fully motorized sample chamber translates in X,Y and Z as well as 360° rotation, which allows optical sectioning from virtually any angle within the specimen. The chamber is equipped for live imaging with control over temperature and CO².
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield, Lightsheet
Microscope Stand:
Sample get introduced vertically into the microscope chamber.
Features:
One microscope chamber is larger and especially designed for holding media used for sample tissue clearing ; Camera: PCO edge 4.2 sCMOS
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 647 nm
Excitation/Emission Filters:
DAPI (Zeiss 49) ; GFP (Zeiss 38HE) ; DsRed (Zeiss 43HE) ; Cy5 (Zeiss 50)
Detector:
Camera
Imaging lenses:
Illumination: 5x Multi Immersion ; 10x Multi Immersion
Detection: 5x Multi Immersion ; 10x (Zeiss loan) ; 20x Multi Immersion
Microscope Control software:
ZenBlack
Temperature Control:
Yes
CO2 Control:
Yes
Humidity Control:
Yes
Light Tight:
Yes
Sample mounting requirements/constraints:
Microscope chamber is virtually built around your sample, with its holder designed to best support your experiment's purpose. Sample holder interface is open both to custom design and machining and 3D printing of your own sample holder.
How to list instrument for inclusion in papers, reports and grant proposals:
The ZEISS Lightsheet 7 was supported by the Howard Hughes Medical Institute.
Location - Building and Room:
Loeb G58
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$35/hr (after 6hrs $17.50/hr)
diSPIM microscope
Microscope Name:
diSPIM
General Overview:
This microscope offers low photo-toxicity and large specimen handling of dual inverted selective plane illumination microscopy (diSPIM) with the power and flexibility of a live-cell microscope system. This system is  suitable for imaging samples in aqueous media.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
CMF, Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Lightsheet
Microscope Stand:
Custom designed for upright applications
Features:
Suitable for imaging large samples at high volumetric speed.
Laser Wavelengths
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter for 405/488/561/640 lasers.
Detector:
Camera
Imaging lenses:
14x, 0.4 NA Multi-immersion lenses; 23x, 0.7 NA Multi-immersion lenses
Microscope Control software:
MicroManager
Temperature Control:
Yes
CO2 Control:
No
Humidity Control:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room
Lillie 215
Contact person:
Louie Kerr, Carsten Wolff, Abhishek Kumar
Hourly Rate for Use:
$33/hr (after 6hrs $16.50/hr)
Zeiss Axio Imager.M2 Microscope
Microscope Name:
Zeiss Axio Imager.M2
General Overview:
Upright compound microscope set up for transmitted light techniques such as brightfield, phase and DIC, and also set up for epi-fluorescence with a X-Cite light source. It includes a motorized stage and Zeiss AxioCams (color and b/w).
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield
Microscope Stand:
Upright
Features:
Epifluorescence ; Cameras: Zeiss AxioCam 503 (b/w) ; Zeiss AxioCam 305 (color)
Excitation/Emission Filters:
DAPI (Zeiss 49) ; AF488 (Zeiss 09) ; GFP (Zeiss 38HE) ; DsRed (Zeiss 43HE) ; Cy5 (Zeiss 50)
Detector:
Camera
Imaging lenses:
5x ; 10x ; 20x ; 63xOil ; 63xW ; 100xOil (more lenses available)
Microscope Control software:
ZenBlue3.1
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Additional details:
Location - Building and Room:
Lillie 209
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$12/hr (after 6hrs $6/hr)
Observer M25 Microscope
Microscope Name:
Zeiss Axio Observer / M25
General Overview:
The Zeiss Axio Observer is set up for transmitted light techniques such as brightfield, phase and DIC and epi-fluorescence with a Colibri LED illuminator and mercury halide lamp. It includes a motorized stage and a Zeiss AxioCam 702.  
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield
Microscope Stand:
Inverted
Features:
M25 multi-focus microscope attached (uses separate cameras to capture simultaneously images from 25 focal planes at different depths in a sample.
Excitation/Emission Filters:
DAPI (Zeiss 49) ; GFP (Zeiss 38HE) ; DsRed (Zeiss 43HE)
Detector:
Camera
Imaging lenses:
2.5x ; 5x ; 10x ; 20x ; 40xOil ; 63xOil / Optovar 1x ; 1.6x
Microscope Control software:
ZenBlue3.4
Temperature Control:
Yes
CO2 Control:
Yes
Humidity Control:
Yes
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Additional details:
Location - Building and Room:
Loeb G61
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$12/hr (after 6hrs $6/hr)
Zeiss Axio Zoom V16 Stereo Microscope
Microscope Name:
Zeiss Axio Zoom V16 Stereo Microscope
General Overview:
The Zeiss Axio Zoom V16 is a stereomicroscope that features a very high numerical aperture over a large field of view. This allows for bright fluorescence imaging of samples from 7x-112x magnification. It is a completely motorized stereomicroscope with automated Z and stage for 3D imaging as well as tiling that combine the highest magnification with the largest field of view.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield
Microscope Stand:
Upright
Features:
Range of magnification : 7x-112x
Equipped with Apotome2
Cameras: Zeiss AxioCam 712 (b/w) ; Zeiss AxioCam 512 (color)
 
Excitation/Emission Filters:
DAPI (Zeiss 49) ; GFP (Zeiss 38HE) ; DsRed (Zeiss 43HE), Cy5 (Zeiss50)
Detector:
Camera
Imaging lenses:
 
Microscope Control software:
ZenBlue3.4
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Additional details:
Location - Building and Room
Lillie 210
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$12/hr (after 6hrs $6/hr)
Zeiss Axio Zoom V12 Stereo Microscope
Microscope Name:
Zeiss Axio Zoom V12 Stereo Microscope
General Overview:
The Discovery V12 is a stereo dissecting microscope set up for transmitted light techniques. It includes a VisiLED base.
Commercial or Pre-commercial:
Commercial
Which Facility:
CMF
Imaging Mode:
Optical
If Optical:
Widefield
Microscope Stand:
Upright
Features:
Equipped with a VisiLED base
Camera: Zeiss AxioCam MRc
Detector:
Camera
Microscope Control software:
AxioVision
Temperature Control:
No
CO2 Control:
No
Humidity Control:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room
Lillie 210
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$12/hr (after 6hrs $6/hr)

Other Equipment

Formlabs and Makerbot 3D printer
Equipment Name:
3D printer Formlabs Form 3B+
General Overview:
Formlabs Form 3B+ is an industrial-quality desktop 3D printer that can produce functional, high-quality prototypes and end-use parts in record time. The Form 3+ offers reliable print quality and accuracy, easy setup and maintenance, and a wide range of high-performance materials.
Commercial or Re-commercial:
Commercial
Which Facility:
CMF
Features:
- Printing resolution 25-300μm (resin layer thickness);
- Build volume 14.5 × 14.5 × 18.5 cm (5.7 × 5.7 × 7.3 in);
- Printing resins available in: black, grey, clear, clear bio-compatible , flexible80A / more available by request;
- FormWash and FormCure for post-processing;
- Workstation with essential software;
Location - Building and Room:
Lillie 215
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$9/10ml used resin
Formlabs and Makerbot 3D printer
Equipment Name:
3D printer MakerBot Replicator2
General Overview:
The MakerBot Replicator2 is a  filament printer (Fused Filament Fabrication) with a resolution of up to 0.1mm.
Commercial or Re-commercial:
Commercial
Which Facility:
CMF
Features:
- Print resolution 0.1 - 0.34 mm;
- Maximum build volume is 11.2 L x 6.0 W x 6.1 H in (28.5 x 15.3 x 15.5 cm);
- PLA filament (diameter: 1.75 mm [0.069 in]) in different colors / more available by request
- Workstation with essential software
Additional details:
Location - Building and Room:
Lillie 215
Contact person:
Louie Kerr, Carsten Wolff
Hourly Rate for Use:
$9/10mm3 printed volume
Image Analysis lab
Equipment Name:
Image Analysis Laboratory
General Overview:
In 2020, the ǧƵ launched its Image Analysis Laboratory to meet the computational needs for imaging and analysis. The Image Analysis Laboratory facilitates several high-end workstations and a GPU server to researchers and course students for data handling and analysis. Additionally, the Lab houses various commercial analysis software and custom designed software for image analysis.
Commercial or Re-commercial:
Commercial
Which Facility:
CMF; Imaging Innovation Initiative
Features:
Installed Software:
Imaris 9.5.1
Avia 11
Arivis
ZEN Blue 3.6
MATLAB
Fiji / ImageJ
Napari
Location - Building and Room:
Lillie 227
Contact person:
Louie Kerr, Carsten Wolff, Abhishek Kumar
Hourly Rate for Use:
N/A